107 papers
This study demonstrates that conventional reference genes are unstable during fish oogenesis and proposes a more robust normalization framework using multiple reference genes and total cDNA concentration to ensure accurate and reproducible transcriptomic analysis in fish ovaries.
This study demonstrates that the AI-enforced PyRMD2Dock platform successfully overcomes computational bottlenecks in ultra-large virtual screening to discover potent, submicromolar small-molecule binders of the challenging immune receptor CD28, which effectively disrupt its interaction with CD80 and inhibit cytokine secretion in human models.
This study demonstrates that plant-derived virus-like nanoparticles displaying the Liprin-1 peptide, when administered via a specific intravenous dosing regimen, effectively induce antigen-specific immune tolerance and regulatory T cell responses to treat rheumatoid arthritis.
This study establishes the first reliable quantitative dose-response relationships for mutagenic O6-HE-dG and non-mutagenic N7-HE-G DNA adducts across multiple tissues in mice exposed to a wide range of ethylene oxide concentrations, revealing distinct low-dose linearity and high-dose disproportionality that inform cancer risk assessment and genotoxic hazard characterization.
This study demonstrates that ethylene oxide induces a hockey-stick-shaped dose-response for genotoxicity in mice, primarily at high concentrations, supporting the biological plausibility of using a single linear slope model for cancer risk assessment under a mutagenic mode of action.
This study demonstrates that intravenous administration of the metalloproteinase inhibitor Marimastat, either alone or combined with a phospholipase A2 inhibitor, significantly improves skeletal muscle regeneration and reduces fibrosis in mice even when given 24 hours after Bothrops asper venom-induced myonecrosis.
This study demonstrates that polystyrene nanoplastics accumulate in the murine cortex and induce size-dependent, transient microglial activation and transcriptomic reprogramming via endolysosomal retention, with 500 nm particles causing significantly more extensive gene expression changes than 100 nm particles.
This study demonstrates that incorporating literature-derived priors into Bayesian hierarchical modeling enables a more robust and physiologically consistent population pharmacokinetic characterization of ceftolozane-tazobactam in critically ill patients with small sample sizes, overcoming the limitations of traditional nonlinear mixed-effects modeling to better optimize dosing strategies.
This preclinical study demonstrates that administering Ifosfamide at 13 Hours After Light Onset (HALO) in mice significantly minimizes multi-organ toxicity, including encephalopathy, by aligning treatment with the drug's circadian tolerance peak.
This study demonstrates that the ethanolic extract of *Asparagus racemosus* exhibits significant antidiabetic potential by inhibiting carbohydrate digestion and absorption, enhancing glucose uptake, and displaying antioxidant activity, likely due to its rich content of phytochemicals such as flavonoids and saponins.
This study demonstrates that microcystin-LR accumulates in human placental trophoblasts via OATP4A1-mediated transport, a process significantly enhanced by both hypoxic conditions and trophoblast cell fusion.
This study demonstrates that an integrated evaluation framework combining structural, functional, and cellular immunotoxicity endpoints in rats effectively characterizes immune system perturbation by showing concordant evidence of lymphoid depletion, impaired adaptive immune responses, and specific lymphocyte subset reductions induced by a reference immunosuppressive compound.
This paper presents an optimized protocol that enables the generation of high-purity, sex-defined primary astrocyte cultures from a single neonatal mouse brain by combining mechanical removal of oligodendrocyte progenitors with 48-hour pharmacological depletion of microglia using the CSF1R inhibitor PLX5622, thereby eliminating the need for tissue pooling and preserving individual genotype and sex for specific biological studies.
This study demonstrates that a microencapsulated extract of *Vaccinium floribundum*, rich in bioactive polyphenols, effectively promotes angiogenesis and attenuates inflammation in both endothelial cell cultures and a mouse edema model, supporting its potential as a therapeutic agent for vascular and inflammatory disorders.
This study comprehensively benchmarks machine learning, deep learning, and transformer models for predicting nuclear receptor activity across 43 Tox21 datasets, revealing that tree-based algorithms excel with highly imbalanced data while deep learning models are more robust for moderate imbalance, and identifying that misclassifications often stem from structurally isolated chemicals lacking training analogues.
This study identifies the evolutionarily conserved age-related decline of the tRNA modification mannosyl-queuosine (manQ) as a critical driver of aging through impaired translational fidelity and proteostasis, demonstrating that supplementation with its precursor, queuine, reverses these defects and extends both lifespan and healthspan across multiple species.
This study demonstrates that the loss of Lamp2a-dependent chaperone-mediated autophagy drives dry AMD-like retinal pathology in mice, which can be effectively rescued by pharmacological activation of BK channels with GLA-1-1 to restore autophagic flux and compensate for CMA deficiency.
This study demonstrates that aerosolized nanoalgosomes derived from the marine microalga *Tetraselmis chuii* are non-cytotoxic, preserve epithelial barrier integrity, and effectively mitigate oxidative stress and inflammation in bronchial epithelial-macrophage co-cultures, highlighting their potential as a safe and sustainable therapeutic strategy for chronic inflammatory lung diseases.
This study comprehensively maps the structure-activity relationships of the antitubercular cyclic peptide griselimycin through systematic chemical modifications and cellular imaging, identifying key molecular determinants essential for its activity to guide the rational design of future tuberculosis therapeutics.
This study reports the development of highly selective, BODIPY-tagged, quenched activity-based probes utilizing a β-lactam warhead that effectively target and visualize Human Neutrophil Elastase in both cell lysates and live human neutrophils, offering new potential for investigating the enzyme's role in cancer.